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ELISA Kit DIY Materials for Progesterone (PG)

P4; Pregn-4-Ene-3,20-Dione

  • Product No.KSA459Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity0.47ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1433  US$ 2388  US$ 4298  US$ 8358  US$ 14328 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Progesterone (PG). No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
Am J Obstet Gynecol. Prevention of preterm birth by progestational agents: what are the molecular mechanisms? Pubmed: 23433326
Placenta The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells Pubmed: 23966103
Veterinary Immunology and Immunopathology Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626
Clinical and Experimental Hypertension Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507
Scientific Reports The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Molecular Neurobiology Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. pubmed:27730513
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Interact Cardiovasc Thorac Surg Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666
Life Sciences The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats pubmed:28599864
Placenta Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy 10.1016:j.placenta.2017.12.004
Nanotheranostics The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Medical Science Monitor Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Pubmed:29674607
Oncology Letters Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … 10.3892:ol.2018.9165
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
Journal of Cellular Physiology Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Pubmed: 30671954
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
Experimental Neurology Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Pubmed: 31377402
Molecular reproduction and development Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries Pubmed: 31490589
Journal of Ovarian Research Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938
Biosensors & Bioelectronics An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesterone Pubmed: 32339148
JOURNAL OF MOLECULAR NEUROSCIENCE Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment Pubmed: 33159671
Chemosphere Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727
ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043
The Journal of Physiology Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Brännström7, Linus R Shao2 … Pubmed: 31206177
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488
DOMESTIC ANIMAL ENDOCRINOLOGY Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells Pubmed: 32739762
Journal of Reproductive Immunology Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and … Pubmed: 32846354
Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal …
Ecotoxicol Environ Safe A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡­ 33862428
Life Sciences MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl 34273377
Antioxidants (Basel) Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer 34439574
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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KSA459Ge11 ELISA Kit DIY Materials for Progesterone (PG) Main materials for "Do It (ELISA Kit) Yourself".