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ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH)
Thyrotropin
- Product No.KSA463Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range185.2-15000pg/mL
- Applicable Sensitivity74.5pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1231
US$ 2052
US$ 3694
US$ 7182
US$ 12312
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH). No significant cross-reactivity or interference between Thyroid Stimulating Hormone (TSH) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Blood | Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID Pubmed: 22184407 |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source |
| Thyroid | Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function Pubmed: 23819433 |
| Journal of Functional Foods | Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats Sciencedirect:S1756464615000894 |
| J Endocrinol. | Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis Pubmed:24928937 |
| journal of lipid research | Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver PubMed: 25713102 |
| Int J Clin Exp Med | Lead exposure causes thyroid abnormalities in diabetic rats PubMed: 26221254 |
| Jubilant Drax Image | Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain Pubmed:27231981 |
| PLoS One. | The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats Pubmed:27043135 |
| journal of bone and mineral research | Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. pubmed:27589370 |
| Toxicological Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384 |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267 |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789 |
| International Journal of Peptide Research and Therapeutics | Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins article:10.1007 |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9 |
| 30 | The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. pubmed:28546511 |
| NITRIC OXIDE-BIOLOGY AND CHEMISTRY | Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidism Pubmed:29307633 |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625 |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
| Neuroscience Letter | Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats Pubmed:29574218 |
| Journal of Neuroinflammation | Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice Pubmed: 30373627 |
| Cell Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008 |
| Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice |
| Endocrine | Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity Pubmed: 31056723 |
| Oxidative Medicine and Cellular Longevity | Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of … |
| Reproductive Biology and Endocrinology | Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice Pubmed: 31729993 |
| Gli | Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice Pubmed: 32173924 |
| Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice Pubmed: 31982645 |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714 |
| Thyroid | Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation Pubmed: 32924834 |
| Thyroid | Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice Pubmed: 32907517 |
| Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy Pubmed: 32442899 |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa … |
| Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyroïdie après 28 jours de traitement au PTU par voie alimentaire. | |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation 33433934 |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart 33375430 |
| Biomedicines | Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131 33572949 |
| Mol Neurobiol | Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway 33966253 |
| Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism 34092241 |
| Pharmaceutics | Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays |
| BRITISH JOURNAL OF NUTRITION | Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats 34250878 |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector 34599290 |
| NatCommun | Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis 34876572 |
| Int J Mol Sci | The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism Pubmed:35163456 |
| Molecular Biology Reports | Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in rats Pubmed:35332411 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
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| CEA463Ra | ELISA Kit for Thyroid Stimulating Hormone (TSH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA463Ra | Multiplex Assay Kit for Thyroid Stimulating Hormone (TSH) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA463Ra11 | ELISA Kit DIY Materials for Thyroid Stimulating Hormone (TSH) | Main materials for "Do It (ELISA Kit) Yourself". |