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ELISA Kit DIY Materials for Cystatin A (CSTA)

Cys-A; STF1; STFA; Stefin A; Cystatin-AS

  • Product No.KSA476Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity25.9pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin A (CSTA). No significant cross-reactivity or interference between Cystatin A (CSTA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Virology Imiquimod Suppresses Propagation of Herpes Simplex Virus 1 by Upregulation of Cystatin A via the Adenosine Receptor A1 Pathway Asm: Source
Scientific Reports Increased levels of the megakaryocyte and platelet expressed cysteine proteases stefin A and cystatin A prevent thrombosis Pubmed: 31270351
International Journal of Reproductive Medicine Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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APA476Mu01 Active Cystatin A (CSTA) Cell culture; Activity Assays.
PAA476Mu01 Polyclonal Antibody to Cystatin A (CSTA) WB; IHC; ICC; IP.
PAA476Mu02 Polyclonal Antibody to Cystatin A (CSTA) WB; IHC; ICC; IP.
LAA476Mu81 FITC-Linked Polyclonal Antibody to Cystatin A (CSTA) WB; IHC; ICC; IF.
LAA476Mu71 Biotin-Linked Polyclonal Antibody to Cystatin A (CSTA) WB; IHC; ICC.
SEA476Mu ELISA Kit for Cystatin A (CSTA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA476Mu Multiplex Assay Kit for Cystatin A (CSTA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA476Mu01 ELISA Kit DIY Materials for Cystatin A (CSTA) Main materials for "Do It (ELISA Kit) Yourself".