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ELISA Kit DIY Materials for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- Product No.KSA482Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range6.17-500pg/mL
- Applicable Sensitivity2.71pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1231
US$ 2052
US$ 3694
US$ 7182
US$ 12312
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness APS: source |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source |
| 36 | Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 PubMed: PMC3695508 |
| Clinics (Sao Paulo). | Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice Pubmed: 24212843 |
| Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829 |
| Transpl Int. | One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys Pubmed:24977654 |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337 |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880 |
| Journal of Surgical Research | Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning Sciencedirect:S0022480414004909 |
| Pharmaceutical Biology | The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil PubMed: 26023989 |
| A Periodical of the Hungarian Academy of Sciences | The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients PubMed: 26690031 |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880 |
| Human & Experimental Toxicology | An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury PubMed: 26078283 |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome science:article |
| Transplant proc | Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. pubmed:27496444 |
| Biomed Journal | Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. pubmed:27884380 |
| International Journal of Advanced Research in Biological Sciences | Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose pdfcopy:42552 |
| Journal of Drug Metabolisn& Toxicology | Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose 587e5b6508aed3826af45e2d.pdf |
| Polish Journal of Veterinary Sciences | Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema pubmed:27760029 |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome article:S1872-9312(16)30085-0 |
| Journal of Molecular Biomarkers J & Diagnosis | Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses DOI: 10.4172/2155-9929.1000318 |
| A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. AN=123819331 | |
| Journal of Functional Foods | Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation 10.1016/j.jff.2017.05.033 |
| Radiation Research | Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute … Pubmed:29809108 |
| Scandinavian Journal of Clinical and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow Pubmed:29301417 |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … Pubmed: 30359461 |
| Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783 |
| Molecular Nutrition & Food Research | Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … Pubmed: 30570214 |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation Pubmed: 31074159 |
| Eurasian Journal of Clinical Sciences | APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735 |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats Pubmed: 33133215 |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency 34119534 |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti … 34736077 |
| J Ethnopharmacol | Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells 34752899 |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats 34343648 |
| Optical Technologies for Biology and Medicine | Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA482Ra01 | Recombinant Endothelin 1 (EDN1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA482Ra01 | Active Endothelin 1 (EDN1) | Cell culture; Activity Assays. |
| CPA482Ra11 | BSA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. |
| CPA482Ra21 | OVA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. |
| PAA482Ra08 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| PAA482Ra01 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| LAA482Ra81 | FITC-Linked Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IF. |
| LAA482Ra71 | Biotin-Linked Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC. |
| MAA482Ra21 | Monoclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| CEA482Ra | ELISA Kit for Endothelin 1 (EDN1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA482Ra | Multiplex Assay Kit for Endothelin 1 (EDN1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA482Ra11 | ELISA Kit DIY Materials for Endothelin 1 (EDN1) | Main materials for "Do It (ELISA Kit) Yourself". |