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ELISA Kit DIY Materials for Fibrinogen Like Protein 2 (FGL2)

T49; PT49; Fibroleukin

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 2 (FGL2). No significant cross-reactivity or interference between Fibrinogen Like Protein 2 (FGL2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Gut. Expression of B and T lymphocyte attenuator (BTLA) in macrophages contributes to the fulminant hepatitis caused by murine hepatitis virus strain-3 PubMed: 22637698
Hepatogastroenterology. Plasma fibrinogen-like protein 2 levels in patients with non-alcoholic fatty liver disease. PubMed: 22024080
PLoS Pathogens Programmed Death (PD)-1-Deficient Mice Are Extremely Sensitive to Murine Hepatitis Virus Strain-3 (MHV-3) Infection PubMed: PMC3131267
Immunology letters Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. Pubmed: 24286726
Immunol Lett. Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection Pubmed:24286726
NATURE COMMUNICATIONS VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism pubmed:29109438
Cancer Medicine Soluble fibrinogen‐like protein 2 ameliorates acute rejection of liver transplantation in rat via inducing Kupffer cells M2 polarization Pubmed:29749104
BMC Infectious Diseases Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases Pubmed: 30419833
Int J Gynaecol Obstet Tandem mass tag©\based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device 33354778
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