ELISA Kit DIY Materials for Oxidized Low Density Lipoprotein (OxLDL)
OL
- Product No.KSA527Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity13.9pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1692
For more details, please contact local distributors! US$ 2820 US$ 5076 US$ 9870 US$ 16920
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Oxidized Low Density Lipoprotein (OxLDL). No significant cross-reactivity or interference between Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
Journal of Ethnopharmacology | Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression ScienceDirect: S0378874109005091 |
Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats ScienceDirect: S1566070211004152 |
Cell Biochemistry and Biophysics | Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients SpringerLink: u466114p03434m6t |
Cardiovascular Research | Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice PubMed: PMC3307381 |
Atherosclerosis | 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits. Pubmed: 24267247 |
PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367 |
Atherosclerosis.? | Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase Pubmed:25549319 |
J Surg Arts | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Cerrahisanatlardergisi:Source |
PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540 |
Saudi Med J | Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptors Pubmed:24888651 |
Vascular Medicine | Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques Pubmed:25516435 |
Journal of Functional Foods | Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice Science: Article |
Acta Pharmacologica Sinica | Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice PubMed: 26592520 |
Cancer Science | Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice PubMed: 26310859 |
BMJ Open Sport Exerc Med | Athletes with higher VO2max present reduced oxLDL after a marathon race Content: 1 |
Biochim Biophys Acta | PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation PubMed: 25728706 |
PLoS One | Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition PubMed: 26555472 |
PLoS One | Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet Pubmed:26790104 |
Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822 |
J Am Heart Assoc. | Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation pmc:PMC4859273 |
Journal of Applied Pharmaceutical Science | Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression admin:php |
Journal of Nutritional Biochemistry | Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice S0955286316301887 |
Journal of Basic and Clinical Physiology and Pharmacology | Effects of altered thyroid states on oxidative stress parameters in rats pubmed:27824612 |
Journal of Functional Foods | Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract S1756464616302511 |
Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218 |
Nutrients | Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen pubmed:29258230 |
Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release pubmed:29146189 |
Journal of Surgical Research | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA 10.14717/jsurgarts.2014.163 |
biorxiv | Significant metabolic improvement by a water extract of olives: animal and human evidence 10.1101/151860 |
Journal of nutritional biochemistry | Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. pubmed:28399420 |
Nutrients | Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice Pubmed:29382111 |
Nutrition & Metabolism | Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western … Pubmed:29755576 |
Journal of Functional Foods | Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties 10.1016:j.jff.2018.07.046 |
Journal of Agricultural and Food Chemistry | Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkers Pubmed:29278909 |
Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet Pubmed:29614743 |
Lipids in Health and Disease | Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice Pubmed:30064511 |
Diabetes Metabolism Research and Reviews | Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats Pubmed:29471582 |
Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians Doi: 10.1016/j.dsx.2018.08.036 |
中国药理学通报 | 三七总皂苷对动脉粥样硬化小鼠的治疗作用 |
Biomedicine & Pharmacotherapy | Effects of lycopene on metabolism of glycolipid in type 2 diabetic rats Pubmed: 30551463 |
Journal of Ethnopharmacology | Anti-diabetic properties of different fractions of Korean red ginseng |
Chinese Pharmacological Bulletin中国药理学通报 | 地昔帕明对兔动脉斑块细胞凋亡及内质网应激的影响 |
Document Version | |
MEDICINE | Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1 Pubmed: 30813134 |
Pharmacological Research | Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent Pubmed: 31181336 |
Oxidative Medicine and Cellular Longevity | Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis |
Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770 |
Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes Pubmed: 31732824 |
Steroids | Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects Pubmed: 31672627 |
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats Pubmed: 32105945 |
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy Axis Pubmed: 31658172 |
Journal of Diabetes Research | Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2A Pubmed: 32280716 |
Acta Biochim Pol | Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors Pubmed: 33108141 |
Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … Pubmed: 32911180 |
Cell Death & Disease | Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells Pubmed: 32796811 |
Nutrients | Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats Pubmed: 32727012 |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation Pubmed: 32635347 |
Oxidative Medicine and Cellular Longevity | Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics Pubmed: 32566106 |
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillation Pubmed: 32510852 |
Antioxidants | Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice 33233455 |
MOLECULAR NUTRITION & FOOD RESEARCH | Digestive n©\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols 33450108 |
Scientific reports | Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice 33483533 |
Scientific reports | Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice 33907226 |
Journal of Food and Nutrition Research | Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults |
Journal of Food and Nutrition Research | Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women |
Eur J Nutr | The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats 34319428 |
Pediatr Gastroenterol Hepatol Nutr | The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with … 34557400 |
J Mol Cell Cardiol | ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription 34343541 |
J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats Pubmed:34986543 |
Oxid Med Cell Longev | DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis Pubmed:35422896 |
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KSA527Ra01 | ELISA Kit DIY Materials for Oxidized Low Density Lipoprotein (OxLDL) | Main materials for "Do It (ELISA Kit) Yourself". |