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ELISA Kit DIY Materials for Immunoglobulin A (IgA)

  • Product No.KSA546Bo01
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, saliva and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.34ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1361  US$ 2268  US$ 4082  US$ 7938  US$ 13608 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Immunoglobulin A (IgA). No significant cross-reactivity or interference between Immunoglobulin A (IgA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
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international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Poultry Science Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422
Clinical and Vaccine Immunology Specific humoral immune response induced by Propionibacterium acnes can prevent Actinobacillus pleuropneumoniae infection in mice Asm: Source
Clin Vaccine Immunol. Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice Pubmed:24429068
Journal of Proteomics Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection Pubmed:24576640
Food & Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407
Pediatrics Oropharyngeal colostrum administration in extremely premature infants: an RCT. Pubmed:25624376
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Food and Agricultural Immunology Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers 10.1080:09540105.2018.1457013
Probiotics Antimicrob Proteins Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686
SAGE Open Medicine Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes Pubmed:29623201
Nephrology Dialysis Transplantation The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosis Pubmed: 31081025
Current Developments in Nutrition Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19) Pubmed: 31225040
Nutrients. Effects of Paramylon Extracted from Euglena gracilis EOD-1 on Parameters Related to Metabolic Syndrome in Diet-Induced Obese Mice Pubmed: 31330894
International Journal of Biological Macromolecules Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens Pubmed: 32224178
Effect Long-Term Exposure to Different Doses of Lipopolysaccharides on the Intestinal Mucosal Immune Barrier
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Microbiology Spectrum Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows 34494854
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KSA546Bo01 ELISA Kit DIY Materials for Immunoglobulin A (IgA) Main materials for "Do It (ELISA Kit) Yourself".