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ELISA Kit DIY Materials for Aquaporin 1 (AQP1)

AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein,28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

  • Product No.KSA579Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.070ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1159  US$ 1932  US$ 3478  US$ 6762  US$ 11592 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Aquaporin 1, Colton Blood Group (AQP1). No significant cross-reactivity or interference between Aquaporin 1, Colton Blood Group (AQP1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Neuroscience Letters Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patients ScienceDirect: S0304394011012341
Cephalalgia Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertension Pubmed:26853804
Clin Neurol Neurosurg.  Cerebrospinal fluid levels of tumor necrosis factor alpha and aquaporin 1 in patients with mild cognitive impairment and idiopathic normal pressure hydrocephalus Pubmed:27155076
Journal of Neurotrauma Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain Injury Pubmed: 31441374
PLoS One Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus 34597351
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA579Ra01 Recombinant Aquaporin 1 (AQP1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA579Ra01 Polyclonal Antibody to Aquaporin 1 (AQP1) WB; IHC; ICC; IP.
SEA579Ra ELISA Kit for Aquaporin 1 (AQP1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA579Ra Multiplex Assay Kit for Aquaporin 1 (AQP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA579Ra01 ELISA Kit DIY Materials for Aquaporin 1 (AQP1) Main materials for "Do It(ELISA Kit) Yourself"