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ELISA Kit DIY Materials for Aquaporin 4 (AQP4)

HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel

  • Product No.KSA582Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.136ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Aquaporin 4 (AQP4). No significant cross-reactivity or interference between Aquaporin 4 (AQP4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Neuroscience Letters Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patients ScienceDirect: S0304394011012341
Neurology Astrocytic damage is far more severe than demyelination in NMO Neurology: 208
Fluids and Barriers of the CNS Aquaporin-4 expression in the cerebrospinal fluid in congenital human hydrocephalus PubMed: PMC3651869
Neuropeptides Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531
Human & Experimental Toxicology Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino rats Pubmed:26987350
Cephalalgia Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertension Pubmed:26853804
PLoS One Cerebrospinal fluid biomarkers of infantile congenital hydrocephalus. pubmed:28212403
Journal of Neurotrauma Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain Injury Pubmed: 31441374
Basic and Clinical Neuroscience Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926
PLoS One Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus 34597351
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA582Ra01 Recombinant Aquaporin 4 (AQP4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA582Ra01 Polyclonal Antibody to Aquaporin 4 (AQP4) WB; IHC; ICC; IP.
MAA582Ra21 Monoclonal Antibody to Aquaporin 4 (AQP4) WB; IHC; ICC; IP.
LAA582Ra72 Biotin-Linked Monoclonal Antibody to Aquaporin 4 (AQP4) WB; IHC; ICC.
SEA582Ra ELISA Kit for Aquaporin 4 (AQP4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA582Ra Multiplex Assay Kit for Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA582Ra01 Antibody Pair for Aquaporin 4 (AQP4) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA582Ra01 ELISA Kit DIY Materials for Aquaporin 4 (AQP4) Main materials for "Do It (ELISA Kit) Yourself".