ELISA Kit DIY Materials for Malondialdehyde (MDA)
- Product No.KSA597Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range24.69-2,000ng/mL
- Applicable Sensitivity9.15ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1433
For more details, please contact local distributors! US$ 2388 US$ 4298 US$ 8358 US$ 14328
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Malondialdehyde (MDA). No significant cross-reactivity or interference between Malondialdehyde (MDA) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
Cell stress chaperones | Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κB PubMed: 21562784 |
Liver transplantation | Effect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled study PubMed: 21837746 |
Journal of Molecular Neuroscience | Formaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of Rats PubMed: 23108488 |
Hepatogastroenterology | The amelioration of AH by abstinence and the attenuation of oxidative stress PubMed: 21940381 |
Clinical and Experimental Hypertension | High salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading doses PubMed: 21529316 |
AJBM | Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats Ajbm: Source |
Yonsei Med J. | Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. Pubmed: 24142649 |
Cardiovasc Pathol.? | Chronic thromboembolic pulmonary hypertension is not associated with iron overload Pubmed:25449047 |
Bosn J Basic Med Sci. | Oxidative stress status in elite athletes engaged in different sport disciplines Pubmed:Pmc4333958 |
Neurochemical Research | Role of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 Cells Pubmed:25005621 |
Stroke.? | Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats. Pubmed:25013016 |
Mol Med Rep | Metformin alleviates high glucose PubMed: 25760137 |
Mediators Inflamm. | Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831 |
PLOS ONE | BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 Cells PubMed: 25749582 |
Mediators of Inflammation | Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831 |
Sci Rep | Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failure PubMed: 26018291 |
Nutrition | Obstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure, PubMed: 26429654 |
Med Sci Monit | Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and Autophagy Pubmed:27011313 |
BMC Complement Altern Med | Mechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calabura Pubmed:26912079 |
Toxicology Mechanisms and Methods | Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism pubmed:27599793 |
International Journal of Neuropsychopharmacology | Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2 pubmed:27988490 |
Revista de Chimie -Bucharest | Comparative Analysis of Some Antioxidant Markers in Periodontal Disease pdf:MARTU%207%2016.pdf |
打不开网页 | Influence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2 180761 |
Cellular Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753 |
Chinese Journal of Integrative Medicine | Panax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats. pubmed:26712211 |
Experimental Cell Research | Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF. pubmed:27641114 |
Anim Reprod Sci. | Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection. pubmed:28007408 |
Journal of Cancer Research and Treatment | Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines jcrt:5 |
Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688 |
Turkish Journal of Veterinary and Animal Sciences | Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summer 795296 |
Oncotarget | Hydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampus pubmed:29245987 |
Experimental and Therapeutic Medicine | Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1 10.3892/etm.2017.5400 |
Pharmacological Reports | Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder 10.1016/j.pharep.2017.08.008 |
Journal of Pain Research | Sex differences in complex regional pain syndrome type I (CRPS-I) in mice pubmed:28831269 |
Environmental toxicology and pharmacology | Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress pubmed:28654832 |
Frontiers in Microbiology | Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress. pubmed:28702016 |
Ecotoxicology and Environmental Safety | Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil Pubmed:29660615 |
Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336 |
Toxicologic Pathology | Gold Nanoparticle Toxicity in Mice and Rats: Species Differences Pubmed:29742986 |
Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473 |
Molecular Medicine Reports | Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781 |
Molecular Medicine Reports | Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway Pubmed:29620209 |
Oncology Letters | Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … Pubmed:29928398 |
Human and Ecological Risk Assessment: An International Journal | Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails 10.1080:10807039.2018.1460800 |
Experimental and Therapeutic Medicine | Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Pubmed:30116359 |
Biochemistry and Biotechnology Research | Lead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixture 2018/1/17-021.pdf |
BMC Pharmacology and Toxicology | Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis Pubmed:29321061 |
Medical Journal Armed Forces India | Are thalassemia patients oxidatively challenged? 10.1016:j.mjafi.2017.12.002 |
Arquivos Brasileiros de Cardiologia | Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged Adults Pubmed: 30328946 |
Clinical and Translational Oncology | Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1 Pubmed: 30284230 |
BMC Complementary and Alternative Medicine | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910 |
Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783 |
Biochemical and Biophysical Research Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats Pubmed: 30454888 |
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Stem Cells Translational Medicine | Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668 |
Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway |
Examination of Oxidative Stress Markers in Women with Postmenopausal Osteoporosis | |
Acta clinica Croatica | The Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal Cancer |
Journal of Pediatric Surgery | The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury |
European Journal of Biomedical and Pharmaceutical Sciences | European Journal of Biomedical AND Pharmaceutical sciences |
Orphanet journal of rare diseases | The cardiovascular phenotype of adult patients with phenylketonuria Pubmed: 31492166 |
Neuropsychobiology | Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic Factor Pubmed: 31550727 |
Alcohol. | Influence of deficient nutrition on trace element status and antioxidant defense system Pubmed: 1781925 |
Journal of Controlled Release | Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injury Pubmed: 31783047 |
Antioxidants | Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese Rats Pubmed: 31905919 |
Frontiers in Neuroscience | Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus Pubmed: 32218719 |
PLoS One | NRF2 deficiency increases obesity susceptibility in a mouse menopausal model Pubmed: 32045430 |
Medicina | Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary Research Pubmed: 31972987 |
Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366 |
Sci Rep | NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population Pubmed: 33177541 |
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Oxidative Medicine and Cellular Longevity | Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics Pubmed: 32566106 |
Food and Agricultural Immunology | Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler … |
Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study | |
Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats | |
Malaysian Journal of Medicine and Health Sciences | Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring |
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING | The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma |
Toxicology | Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect 33259821 |
Turk J Pharm Sci | Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats 33389951 |
Biosci Rep | Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial 32686821 |
Stem Cell Research & Therapy | Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis 33413675 |
J Pediatr Urol | Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats 33608226 |
Mol Med Rep | Tanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction rats 33786621 |
Amino Acids | Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats 34089389 |
Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats 34093766 |
Experimental and Therapeutic Medicine | MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma 33732346 |
The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation and Oxidative Stress Parameters and Faecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity ¨C PRO-DEMET Randomised Study Protocol 33804999 | |
J Healthc Eng | κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway 34608407 |
Cell Death Discov | Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats Pubmed:35022384 |
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J Inflamm Res | The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats Pubmed:35645576 |
BioMed Research International | Malondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive … Pubmed:35224102 |
Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy | |
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