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ELISA Kit DIY Materials for Pepsin (PP)

  • Product No.KSA632Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range2.47-200ng/mL
  • Applicable Sensitivity0.89ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Pepsin (PP). No significant cross-reactivity or interference between Pepsin (PP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Radiology and Oncology LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMA Szd:Source
Radiology and Oncology Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer Pubmed:Pmc4362607
Clin Otolaryngol Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative study PubMed: 25516364
journal of pediatric gastroenterology and nutrition Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants? PubMed: 25313850
Journal of Voice Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia pubmed:28756936
Sensors (Basel) Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode Pubmed:29882917
The Tohoku Journal of Experimental Medicine Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease
Respiratory Research Pulmonary aspiration in preschool children with cystic fibrosis Pubmed: 30558606
Tanaffos Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease Exacerbation Pubmed: 31423141
Sensors Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux Pubmed: 31935973
BIOSCIENCE REPORTS Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasms Pubmed: 33103719
OTOLARYNGOLOGY-HEAD AND NECK SURGERY Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia Pubmed: 32692278
American Journal of Otolaryngology Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps Pubmed: 32889371
The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients
Med Sci Monit The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour?¡­ 33220027
Annals of Esophagus Pepsin properties, structure, and its accurate measurement: a narrative review
Laryngoscope The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis 34338331
Pulmonology Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia Pubmed:35715334
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CEA632Ra ELISA Kit for Pepsin (PP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA632Ra Multiplex Assay Kit for Pepsin (PP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA632Ra11 ELISA Kit DIY Materials for Pepsin (PP) Main materials for "Do It (ELISA Kit) Yourself".