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ELISA Kit DIY Materials for Toll Like Receptor 9 (TLR9)

CD289

  • Product No.KSA709Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Toll Like Receptor 9 (TLR9). No significant cross-reactivity or interference between Toll Like Receptor 9 (TLR9) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Chinese Medical Journal Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297
DNA Cell Biol. Expression of serum toll-like receptor 9 and oxidative damage markers in benign and malignant breast diseases Pubmed:24906089
Journal of Trace Elements in Medicine and Biology Correlation of serum toll like receptor 9 and trace elements with lipid peroxidation in the patients of breast diseases PubMed: 25744504
Journal of cancer research and clinical oncology TLR9 gene polymorphism− 1486T/C (rs187084) is associated with uterine cervical neoplasm in Mexican female population pubmed:28819773
In Vivo The Relationship Between Sepsis-induced Immunosuppression and Serum Toll-like Receptor 9 Level Pubmed: 30348730
The role of toll-like receptor 9 (TLR9) in Epstein-Barr virus-associated gastric cancer
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA709Mu01 Recombinant Toll Like Receptor 9 (TLR9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA709Mu01 Polyclonal Antibody to Toll Like Receptor 9 (TLR9) WB; IHC; ICC; IP.
SEA709Mu ELISA Kit for Toll Like Receptor 9 (TLR9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA709Mu Multiplex Assay Kit for Toll Like Receptor 9 (TLR9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA709Mu01 ELISA Kit DIY Materials for Toll Like Receptor 9 (TLR9) Main materials for "Do It (ELISA Kit) Yourself".