ELISA Kit DIY Materials for Indole 3 Acetic Acid (IAA)

IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Indole 3 Acetic Acid (IAA). No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Chemosphere Assisted phytoremediation of mixed metal(loid)-polluted pyrite waste: effects of foliar and substrate IBA application on fodder radish PubMed: 21570105
Biology and Fertility of Soils Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens SQR9 Springer:Source
Plant Science Structural and metabolic alterations in root systems under limited water conditions in forage grasses of Lolium-Festuca complex
Nutrients Indole-3-Acetic Acid Alleviates Nonalcoholic Fatty Liver Disease in Mice via Attenuation of Hepatic Lipogenesis, and Oxidative and Inflammatory Stress Pubmed: 31484323
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KSA737Ge11 ELISA Kit DIY Materials for Indole 3 Acetic Acid (IAA) Main materials for "Do It (ELISA Kit) Yourself".