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ELISA Kit DIY Materials for Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

  • Product No.KSA780Bo01
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, saliva, milk,cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.62ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1361  US$ 2268  US$ 4082  US$ 7938  US$ 13608 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Lactoferrin (LTF).No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438
Bioscience, Biotechnology, and Biochemistry Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211
Pulmonary Pharmacology & Therapeutics Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells Pubmed:29627483
Microbial Pathogenesis TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093
Biomedicines Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury Pubmed:35625931
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
NPA780Bo01 Native Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA780Bo01 Recombinant Lactoferrin (LTF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA780Bo05 Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
PAA780Bo01 Polyclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
MAA780Bo21 Monoclonal Antibody to Lactoferrin (LTF) WB; IHC; ICC; IP.
SEA780Bo ELISA Kit for Lactoferrin (LTF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA780Bo CLIA Kit for Lactoferrin (LTF) Chemiluminescent immunoassay for Antigen Detection.
LMA780Bo Multiplex Assay Kit for Lactoferrin (LTF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA780Bo01 ELISA Kit DIY Materials for Lactoferrin (LTF) Main materials for "Do It (ELISA Kit) Yourself".