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ELISA Kit DIY Materials for Amylin

IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

  • Product No.KSA812Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range6.17-500pg/mL
  • Applicable Sensitivity2.51pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Islet Amyloid Polypeptide (IAPP). No significant cross-reactivity or interference between Islet Amyloid Polypeptide (IAPP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
plos ONE Differential Effect of Amylin on Endothelial-Dependent Vasodilation in Mesenteric Arteries from Control and Insulin Resistant Rats PubMed: 25807378
Nutrients Glucagon Shows Higher Sensitivity than Insulin to Grapeseed Proanthocyanidin Extract (GSPE) Treatment in Cafeteria-Fed Rats 33810265
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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KSA812Hu11 ELISA Kit DIY Materials for Amylin Main materials for "Do It (ELISA Kit) Yourself".