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ELISA Kit DIY Materials for Nitric Oxide Synthase 2, Inducible (NOS2)
NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
- Product No.KSA837Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.47-30ng/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1166
US$ 1944
US$ 3499
US$ 6804
US$ 11664
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2).No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice PubMed: 20713153 |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression ScienceDirect: S0925443911001323 |
| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta Wiley: source |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors ScienceDirect: S0378111911007347 |
| PLoS One | A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide PubMed: 23359814 |
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| Journal of Cellular Biochemistry | The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. PubMed: 22234777 |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114 |
| Integrative Cancer Therapies | Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits Pubmed: 24013642 |
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| International Immunopharmacology | Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153 |
| INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH | L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source |
| Molecular Vision | Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats Pubmed:24940038 |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292 |
| Eur J Cancer Prev. | Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies Pubmed:25222193 |
| Cell and Tissue Research | Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes Pubmed:25673209 |
| Ann N Y Acad Sci.? | Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis Pubmed:5243883 |
| Molecular Medicine Reports | Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury PubMed: 26458999 |
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| Cancer Medicine | Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254 |
| Journal of Natural Medicines | Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice Pubmed:26972100 |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496 |
| Медицина и здравоохранение | Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу article:n |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909 |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494 |
| Molecular Vision | Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. pubmed:27440992 |
| Journal of Natural Medicines | Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice. pubmed:26972100 |
| Applied Biochemistry and Biotechnology | Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines. pubmed:27435276 |
| Cell Mol Neurobiol | Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms pubmed:28676988 |
| Microbial Immunology | Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota pubmed:28713364 |
| Biomedicine & Pharmacotherapy | Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines pubmed:28531922 |
| The Royal Society of Chemistry | Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway 10.1039/C7RA08879E |
| Acta Pharmacologica Sinica | Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ. pubmed:28649126 |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects pubmed:29102164 |
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| European Journal of Pharmacology | Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat Pubmed:29518393 |
| Regulatory Toxicology and Pharmacology | Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and … Pubmed:29678767 |
| Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder 10.1016:j.pharep.2018.06.001 |
| Clinical And Experimental Pharmacology And Physiology | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336 |
| Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne | |
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| Metabolic Brain Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms Pubmed: 30421246 |
| PLoS One | Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages Pubmed: 30412620 |
| The Eurasian Journal of Medicine | Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats Doi: 10.5152/eurasianjmed.2018.18160 |
| PLoS One | Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury Pubmed: 30571701 |
| International Journal of Environmental Research and Public Health | Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women Pubmed: 30513890 |
| Journal of Functional Foods | Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments |
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| PLoS One | Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients 33370317 |
| Biotechnol Rep | Targeting NF-¦ÊB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro 33598414 |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926 |
| Fermented Foods and Microbes Related to Health | Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A¦Â40 and Streptozotocin?¡ |
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| Drug Chem Toxicol | Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline … 34167392 |
| Frontiers in Cardiovascular Medicine | The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome 34513946 |
| Nutrients | Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice 34684662 |
| biomolecules | Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men 34356664 |
| Hum Exp Toxicol | Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat 34155936 |
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