ELISA Kit DIY Materials for Thyrotropin Releasing Hormone (TRH)
TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
- Product No.KSA839Mu11
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range6.17-500pg/mL
- Applicable Sensitivity2.39pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1166
For more details, please contact local distributors! US$ 1944 US$ 3499 US$ 6804 US$ 11664
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Thyrotropin Releasing Hormone (TRH). No significant cross-reactivity or interference between Thyrotropin Releasing Hormone (TRH) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
Plos one | Thyrotropin-Releasing Hormone (TRH) Promotes Wound Re-Epithelialisation in Frog and Human Skin PubMed: PMC3759422 |
Analyst | Ultrasensitive detection of thyrotropin-releasing hormone based on azo coupling and surface-enhanced resonance Raman spectroscopy Pubmed:27338554 |
journal of neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder. pubmed:28199771 |
Journal of Neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder 10.1111/jne.12461 |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
Cell Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008 |