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ELISA Kit DIY Materials for Apelin 12 (AP12)

APLN12

  • Product No.KSA856Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range74.07-6000pg/mL
  • Applicable Sensitivity26.52pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 804  US$ 1340  US$ 2412  US$ 4690  US$ 8040 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Apelin 12 (AP12). No significant cross-reactivity or interference between Apelin 12 (AP12) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clinical and Experimental Hypertension Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3 Pubmed:29652188
Pediatr Diabetes Adropin and apelin‐12 efficiently predict metabolic syndrome in obese children Pubmed: 32749012
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
KSA856Ra11 ELISA Kit DIY Materials for Apelin 12 (AP12) Main materials for "Do It (ELISA Kit) Yourself".