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ELISA Kit DIY Materials for Parathyroid Hormone (PTH)

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

  • Product No.KSA866Ga01
  • Organism SpeciesChicken (Gallus) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.070ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1189  US$ 1981  US$ 3566  US$ 6934  US$ 11886 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Biological trace element research Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet PubMed: 19915804
Basic nutritional investigation Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice ScienceDirect: S0899900709004808
The Korean Journal of Nutrition Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats KoreaMed: source
Acta Physiologica Hungarica Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters Pubmed: 22173025
Toxicology and Industrial Health The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
Indian Journal of Experimental Biology Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. PubMed: 22439438
Plos one Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? PubMed: PMC3679169
Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
Clinical Cancer Research Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source
Nutricion Hospitalaria Effect of the “protein diet” and bone tissue Aulamedica: Source
Academic Journal Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source
J Proteome Res. Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets Pubmed:25051233
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
J Nutr Sci Vitaminol (Tokyo) The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats PubMed: 26639847
The Korean Society of Pharmacognosy Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat Article: Hksobf_2015_V46N2_167
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
7 Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908
PLOS ONE Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. pubmed:27442254
International Journal of Advanced Research in Biological Sciences Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018
American Journal of Physiology-Renal Physiology Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice Pubmed:29363324
Tropical Animal Health and Production Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism Pubmed:29931604
International Immunopharmacology CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts Pubmed: 31679847
ENDOCRINOLOGY A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats Pubmed: 32852547
Kidney International Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts.
PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES
Oxid Med Cell Longev Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats 34373766
Gene Therapy Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector 34599290
Cells Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury Pubmed:35456008
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
RPA866Ga01 Recombinant Parathyroid Hormone (PTH) Positive Control; Immunogen; SDS-PAGE; WB.
PAA866Ga01 Polyclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC; IP.
LAA866Ga71 Biotin-Linked Polyclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC.
LAA866Ga81 FITC-Linked Polyclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC; IF.
KSA866Ga01 ELISA Kit DIY Materials for Parathyroid Hormone (PTH) Main materials for "Do It(ELISA Kit) Yourself"