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ELISA Kit DIY Materials for Calcitonin Gene Related Peptide (CGRP)

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

  • Product No.KSA876Mu11
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.68pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1037  US$ 1728  US$ 3110  US$ 6048  US$ 10368 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Evidence-Based Complementary and Alternative Medicine Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. Hindawi: 190370
The Journal of Headache and Pain Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker PubMed: PMC3620076
Headache: The Journal of Head and Face Pain CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372
PLoS One. Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A Pubmed:Pmc4028234
Int J Vasc Med. Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. Pubmed:24624298
Arch Gynecol Obstet. Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study Pubmed:24943060
Pain OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine PubMed: 25735000
Molecular Medicine Reports Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats PubMed: 25892548
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233
Cardiology.  Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. Pubmed:27144592
J Headache Pain Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB Pubmed:27150105
Frontiers in Physiology Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition. pubmed:27445864
Naunyn Schmiedebergs Arch Pharmacol. Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. pubmed:27896372
American Journal of Translational Research A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979
9 Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome pubmed:28483500
Journal of Clinical Investigation CNS myeloid cells critically regulate heat hyperalgesia Pubmed:29634489
Journal of Headache and Pain Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisal Pubmed:30006780
BioRxiv - the preprint server for biology RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis 10.1101:357582
International Journal of Pharmaceutical Sciences and Research MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
JOURNAL OF PERIODONTOLOGY Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine Pubmed: 31070784
Molecular and Cellular Biochemistry Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling Pubmed: 31420791
Journal of Headache and Pain Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455
Europe PMC Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion … Pubmed: 31375062
Clinical Chemistry and Laboratory Medicine Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache Pubmed: 32286239
MOLECULAR BIOLOGY REPORTS Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice Pubmed: 32951099
Scientific Reports Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs 33441852
Nature Communications Perirenal adipose afferent nerves sustain pathological high blood pressure in rats Pubmed:35668093
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA876Mu02 Recombinant Calcitonin Gene Related Peptide (CGRP) Positive Control; Immunogen; SDS-PAGE; WB.
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CPA876Mu21 OVA Conjugated Calcitonin Gene Related Peptide (CGRP) Immunogen; SDS-PAGE; WB.
PAA876Mu02 Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP) WB; IHC; ICC; IP.
PAA876Mu01 Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP) WB; IHC; ICC; IP.
CEA876Mu ELISA Kit for Calcitonin Gene Related Peptide (CGRP) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA876Mu11 ELISA Kit DIY Materials for Calcitonin Gene Related Peptide (CGRP) Main materials for "Do It (ELISA Kit) Yourself".