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ELISA Kit DIY Materials for Gamma-Aminobutyric Acid (gABA)

4-Aminobutyric Acid

  • Product No.KSA900Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2000pg/mL
  • Applicable Sensitivity9.27pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1211  US$ 2019  US$ 3634  US$ 7067  US$ 12114 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Gamma-Aminobutyric Acid (gABA). No significant cross-reactivity or interference between Gamma-Aminobutyric Acid (gABA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Toxicology and Industrial Health Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum PubMed: 21444355
Neurosci Lett Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. PubMed: 18577424
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
Scientific Reports Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension pubmed:27917928
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894
Scientific Reports A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme Pubmed: 30341396
Neurochemical Research Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission Pubmed: 30666488
Neuroscience Letters Differentiation of human glioblastoma U87 cells into cholinergic neuron Pubmed: 30928478
Neurochemical Journal Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism
Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.
Experimental and Therapeutic Medicine Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy Pubmed: 33101467
Exp Anim Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation 33828024
Neurogenetics Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases 34101060
Sci Rep Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission 34475508
J Food Biochem Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies Pubmed:35102569
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA900Ge11 BSA Conjugated Gamma-Aminobutyric Acid (gABA) Immunogen; SDS-PAGE; WB.
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KSA900Ge11 ELISA Kit DIY Materials for Gamma-Aminobutyric Acid (gABA) Main materials for "Do It (ELISA Kit) Yourself".