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ELISA Kit DIY Materials for Secretin (SCT)

  • Product No.KSB075Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.83pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1241  US$ 2069  US$ 3724  US$ 7242  US$ 12414 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Secretin (SCT). No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
SpringerPlus Weak cation exchange magnetic beads coupled with matrix-assisted laser desorption ionization-time of flight-mass spectrometry in screening serum protein markers in osteopenia Pubmed:27347465
Cell Secretin-Activated Brown Fat Mediates Prandial Thermogenesis to Induce Satiation Pubmed: 30449620
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
Novel non-adrenergic, endocrine mediators of brown adipose tissue thermogenesis
Nat Metab Secretin activates brown fat and induces satiation 34158656
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CEB075Ra ELISA Kit for Secretin (SCT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB075Ra Multiplex Assay Kit for Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB075Ra11 ELISA Kit DIY Materials for Secretin (SCT) Main materials for "Do It (ELISA Kit) Yourself".