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ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

  • Product No.KSB081Po01
  • Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.61ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1220  US$ 2034  US$ 3661  US$ 7119  US$ 12204 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 1A (HSPA1A).No significant cross-reactivity or interference between Heat Shock 70kDa Protein 1A (HSPA1A) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Neuroinflammation Identification of potential biomarkers of gold nanoparticle toxicity in rat brains PubMed: 22691312
Mediators Inflamm.? Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs Pubmed:Pmc4324980
Eur J Pharmacol. Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats Pubmed:24508522
Pharmacol Rep. Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice Pubmed:25443743
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics pubmed:28822267
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young … Pubmed:29379533
metabolic brain disease Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus Pubmed: 32172517
EXPERIMENTAL CELL RESEARCH Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice Pubmed: 32439493
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPB081Po01 Recombinant Heat Shock 70kDa Protein 1A (HSPA1A) Positive Control; Immunogen; SDS-PAGE; WB.
PAB081Po01 Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC; IP.
MAB081Po21 Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) WB; IHC; ICC; IP.
SEB081Po ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB081Po Multiplex Assay Kit for Heat Shock 70kDa Protein 1A (HSPA1A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB081Po01 ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A) Main materials for "Do It (ELISA Kit) Yourself".