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ELISA Kit DIY Materials for Interleukin 31 (IL31)

  • Product No.KSB179Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.7pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 31 (IL31). No significant cross-reactivity or interference between Interleukin 31 (IL31) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
allergy and clinical immunology Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis Siaic-Review:Source
BMC Immunol Increased levels of interleukin 31 (IL-31) in osteoporosis PubMed: 2644965
Sci Rep. Interleukin-31 expression and relation to disease severity in human asthma pmc:PMC4783779
Exp Dermatol. Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice. pubmed:28191683
Nat Commun.  The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. pubmed:28067314
Experimental Dermatology Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice doi:10.1111
アトピー性皮膚炎の痒みの発症機序に関する研究
Indian Journal of Public Health Research & Development Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)
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KSB179Ra01 ELISA Kit DIY Materials for Interleukin 31 (IL31) Main materials for "Do It (ELISA Kit) Yourself".