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ELISA Kit DIY Materials for Cluster Of Differentiation 73 (CD73)

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

  • Product No.KSB250Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.261ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1189  US$ 1981  US$ 3566  US$ 6934  US$ 11886 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of 5'-Nucleotidase, Ecto (NT5E). No significant cross-reactivity or interference between 5'-Nucleotidase, Ecto (NT5E) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Autoimmunity The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation Pubmed:25640206
Talanta Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … 10.1016:j.talanta.2018.01.044
Cancer Biomarkers Expression and clinical significance of serum NT5E protein in patients with colorectal cancer Pubmed: 30932882
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB250Mu01 Recombinant Cluster Of Differentiation 73 (CD73) Positive Control; Immunogen; SDS-PAGE; WB.
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LAB250Mu81 FITC-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IF.
LAB250Mu71 Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC.
KSB250Mu01 ELISA Kit DIY Materials for Cluster Of Differentiation 73 (CD73) Main materials for "Do It(ELISA Kit) Yourself"