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ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • Product No.KSB966Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.67ng/m
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Syndecan 1 (SDC1). No significant cross-reactivity or interference between Syndecan 1 (SDC1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction SpringerLink: a201w3l18k257462
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats Springer: Source
Atherosclerosis. Damage of the endothelial glycocalyx in chronic kidney disease Pubmed:24727235
Journal of Trauma and Acute Care Surgery Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury Pubmed:25058256
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance Pubmed:25692257
Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding PubMed: 26199464
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. PubMed: 26371596
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury pubmed:27405618
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection pubmed:27643669
Danish National RESEARCH DATABASE Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats 10.1089
Journal of Neurotrauma Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat. doi/10.1089/neu.2016.4574
British Journal of Anaesthesia Volume kinetics of Ringer's lactate solution in acute inflammatory disease 10.1016:j.bja.2018.04.023
Arteriosclerosis, Thrombosis, and Vascular Biology MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge Pubmed:29903730
Shock Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis Pubmed:28591008
Shock Experimental models of endotheliopathy: impact of shock severity Pubmed:28697004
Journal of Trauma and Acute Care Surgery Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat Pubmed:28930945
The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions 173015_06.pdf
Journal of Surgical Research Plasma ameliorates endothelial dysfunction in burn injury Doi: 10.1016/j.jss.2018.08.027
Journal of Anesthesia Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model Pubmed: 30374889
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass Pubmed: 30687934
Journal of Cardiothoracic and Vascular Anesthesial Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit Pubmed: 31787433
Hepatobiliary & Pancreatic Diseases International Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP Pubmed: 31919038
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis 33375342
Microvasc Res Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency 34119534
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … 34423832
Shock Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke 34524269
Exp Anim Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model Pubmed:35110424
Journal of Emergency Medicine Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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SEB966Ra ELISA Kit for Syndecan 1 (SDC1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB966Ra Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB966Ra01 ELISA Kit DIY Materials for Syndecan 1 (SDC1) Main materials for "Do It(ELISA Kit) Yourself"