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ELISA Kit DIY Materials for Interleukin 33 (IL33)

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

  • Product No.KSB980Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.4pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 33 (IL33). No significant cross-reactivity or interference between Interleukin 33 (IL33) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The Korean Journal of Internal Medicine Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction PubMed: PMC3604606
Acta Histochem CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice PubMed: 26456520
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Journal of Interferon & Cytokine Research The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. pubmed:27348082
Archives of Dermatological Research IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo pubmed:27388717
Sci Pharm. Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
Biotechnology & Biotechnological Equipment  Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) 10.1080:13102818.2018.1471361
Tropical Journal of Pharmaceutical Research Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer 172341
Particle and Fibre Toxicology Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure Pubmed: 30413212
Scientific Reports Interleukin-33 serum levels in postmenopausal women with osteoporosis Pubmed: 30846811
Cell Reports Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s Pubmed: 32101749
Allergy Asthma & Immunology Research Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics Pubmed: 32141256
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus 34395633
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB980Hu02 Recombinant Interleukin 33 (IL33) Positive Control; Immunogen; SDS-PAGE; WB.
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MAB980Hu23 Monoclonal Antibody to Interleukin 33 (IL33) WB; IHC; ICC; IP.
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KSB980Hu01 ELISA Kit DIY Materials for Interleukin 33 (IL33) Main materials for "Do It (ELISA Kit) Yourself".