ELISA Kit DIY Materials for Anterior Gradient 2 (AGR2)

GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog

  • Product No.KSC285Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.62ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1237  US$ 2062  US$ 3712  US$ 7217  US$ 12372 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Cancer Biomarkers Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinoma MetaPress: n7137r6271u54118
Molecular & Cellular Proteomics Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers PubMed: PMC3205865
Prostate. Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotype Pubmed: 22911164
BMC Cancer. Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9 Pubmed: 24007603
Clinical Cancer Research Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic Cancer Pubmed:25239611
PLoS One AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer Detection PubMed: 25875093
Clinical Neurology and Neurosurgery Serum AGR2 as a useful biomarker for pituitary adenomas S0303846717300045
Oncotarget Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2) Pubmed:29937991
Int J Oncol Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape 34913074
International Journal of Cancer Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer Pubmed:35689436
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC285Ra01 Recombinant Anterior Gradient 2 (AGR2) Positive Control; Immunogen; SDS-PAGE; WB.
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MAC285Ra22 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra23 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra26 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra21 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra24 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra27 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
MAC285Ra25 Monoclonal Antibody to Anterior Gradient 2 (AGR2) WB; IHC; ICC; IP.
KSC285Ra01 ELISA Kit DIY Materials for Anterior Gradient 2 (AGR2) Main materials for "Do It(ELISA Kit) Yourself"