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ELISA Kit DIY Materials for Apelin (APLN)

XNPEP2; APEL; APJ endogenous ligand

  • Product No.KSD066Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range19.75-1600pg/mL
  • Applicable Sensitivity8.25pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Apelin (APLN). No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clin Exp Hypertens. Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension Informahealthcare: Source
African Journal of Pharmacy and Pharmacology Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. Academicjournals: Source
Zahedan Journal of research in Medical Sciences Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats Zjrms: Source
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658
Mol Metab TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice PubMed: 26500845
Journal of medicinal food Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. pubmed:28170279
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696
preprints Serum Apelin and Galactin-3 in Preeclampsia in Iraq Pubmed: 32538210
Journal of Diabetes Research The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD066Hu02 Recombinant Apelin (APLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPD066Hu01 Recombinant Apelin (APLN) Positive Control; Immunogen; SDS-PAGE; WB.
SPD066Hu01 Synthetic Apelin (APLN) Immunogen; Blocking Peptide.
CPD066Hu21 OVA Conjugated Apelin (APLN) Immunogen; SDS-PAGE; WB.
PAD066Hu03 Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
PAD066Hu08 Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
PAD066Hu02 Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
PAD066Hu09 Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
PAD066Hu01 Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
LAD066Hu71 Biotin-Linked Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC.
LAD066Hu81 FITC-Linked Polyclonal Antibody to Apelin (APLN) WB; IHC; ICC; IF.
SED066Hu ELISA Kit for Apelin (APLN) Enzyme-linked immunosorbent assay for Antigen Detection.
CED066Hu ELISA Kit for Apelin (APLN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD066Hu Multiplex Assay Kit for Apelin (APLN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD066Hu11 ELISA Kit DIY Materials for Apelin (APLN) Main materials for "Do It(ELISA Kit) Yourself"