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ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

  • Product No.KSD232Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.9pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac (TNNT2). No significant cross-reactivity or interference between Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats PubMed: 21232020
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats Wiley: source
European Journal of Pharmacology Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats ScienceDirect: S0014299909009984
Journal of translational medicine The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury PubMed: PMC3694448
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction Pubmed: 22834943
An International Journal of Pharmaceutical Sciences In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium Pubmed: 23700796
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source
Liver Int. Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis Pubmed:24330504
Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs Pubmed:24420420
British Journal of Pharmacology Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise Pubmed:25039894
Chemico-Biological Interactions Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765
The Journal of Pharmacology and Experimental Therapeuties The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice Pubmed:27247000
The Journal of Maternal-Fetal & Neonatal Medicine Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. pubmed:27937003
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830
PLoS One Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model pubmed:28570599
EXPERIMENTAL AND THERAPEUTIC MEDICINE Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus pubmed:29042932
PLoS One Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury Pubmed: 30192780
PLoS One Tissue suction-mediated gene transfer to the beating heart in mice Pubmed: 32027678
Journal of Human Kinetics Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy Pubmed: 32148580
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535
Toxics Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats Pubmed: 32947820
Elife Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice 33526170
Cell Metabolism Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344
Anal Bioanal Chem Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993
Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD232Ra01 Recombinant Troponin T Type 2, Cardiac (TNNT2) Positive Control; Immunogen; SDS-PAGE; WB.
PAD232Ra01 Polyclonal Antibody to Troponin T Type 2, Cardiac (TNNT2) WB; IHC; ICC; IP.
MAD232Ra21 Monoclonal Antibody to Troponin T Type 2, Cardiac (TNNT2) WB; IHC; ICC; IP.
MED232Ra Mini Samples ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Enzyme-linked immunosorbent assay for Antigen Detection.
SED232Ra ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD232Ra CLIA Kit for Troponin T Type 2, Cardiac (TNNT2) Chemiluminescent immunoassay for Antigen Detection.
LMD232Ra Multiplex Assay Kit for Troponin T Type 2, Cardiac (TNNT2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD232Ra01 ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2) Main materials for "Do It(ELISA Kit) Yourself"