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ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1)

CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

  • Product No.KSD295Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.129ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2167  US$ 3901  US$ 7585  US$ 13002 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cytochrome P450 1A1 (CYP1A1). No significant cross-reactivity or interference between Cytochrome P450 1A1 (CYP1A1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats Pubmed:25733458
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
International Journal of Chemical and Natural Science Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone portal:uploads
International Journal of Pharma Sciences Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues portal:uploads
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
Biochemistry (Mosc) Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells Pubmed:29738693
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPD295Mu01 Recombinant Cytochrome P450 1A1 (CYP1A1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD295Mu01 Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1) WB; IHC; ICC; IP.
KSD295Mu01 ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1) Main materials for "Do It (ELISA Kit) Yourself".