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ELISA Kit DIY Materials for Sirtuin 4 (SIRT4)

SIR2L4; Silent Mating Type Information Regulation 2 Homolog 4; SIR2-like protein 4; NAD-dependent protein deacetylase sirtuin-4

  • Product No.KSE914Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.055ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1207  US$ 2011  US$ 3620  US$ 7039  US$ 12066 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Sirtuin 4 (SIRT4). No significant cross-reactivity or interference between Sirtuin 4 (SIRT4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Growth Preliminary data on the relationship between circulating levels of Sirtuin 4, anthropometric and metabolic parameters in obese subjects according to growth hormone/insulin-like growth factor-1 status PubMed: 25466907
hindawi ADHERENCE TO THE MEDITERRANEAN DIET AND CIRCULATING LEVELS OF SIRTUIN 4 IN OBESE 6101254.pdf
Oxidative Medicine and Cellular Longevity Adherence to the Mediterranean Diet and Circulating Levels of Sirtuin 4 in Obese Patients: A Novel Association pubmed:28706576
Elife SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation Pubmed:29504933
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPE914Mu01 Recombinant Sirtuin 4 (SIRT4) Positive Control; Immunogen; SDS-PAGE; WB.
PAE914Mu01 Polyclonal Antibody to Sirtuin 4 (SIRT4) WB; IHC; ICC; IP.
LAE914Mu71 Biotin-Linked Polyclonal Antibody to Sirtuin 4 (SIRT4) WB; IHC; ICC.
KSE914Mu01 ELISA Kit DIY Materials for Sirtuin 4 (SIRT4) Main materials for "Do It(ELISA Kit) Yourself"