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ELISA Kit DIY Materials for Nicotinamide Adenine Dinucleotide (NAD)

  • Product No.KSG409Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.5-10,000ng/mL
  • Applicable Sensitivity49.3ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1202  US$ 2003  US$ 3605  US$ 7011  US$ 12018 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide (NAD). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide (NAD) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
neurotoxicology Targeting sirtuin activity with nicotinamide riboside reduces neuroinflammation in a GWI mouse model Pubmed: 32343995
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KSG409Ge11 ELISA Kit DIY Materials for Nicotinamide Adenine Dinucleotide (NAD) Main materials for "Do It (ELISA Kit) Yourself".