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ELISA Kit DIY Materials for Calprotectin (CALPRO)

MRP-8/MRP-14; S100A8/A9

  • Product No.KSK504Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range125-2,000pg/mL
  • Applicable Sensitivity50pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Calprotectin (CALPRO). No significant cross-reactivity or interference between Calprotectin (CALPRO) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Medicine (Baltimore) A Novel Combination of Calprotectin and CXCL12 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632726
Neurochemical Research  Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice pubmed:27995494
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. pubmed:28672877
Analytical Chemistry  Multiplex Immuno-MALDI-TOF MS for Targeted Quantification of Protein Biomarkers and Their Proteoforms Related to Inflammation and Renal Dysfunction Pubmed:29420882
 JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH Maternal serum calprotectin level in intrahepatic cholestasis of pregnancy 34254410
Bull Exp Biol Med Influence of Rectal Ozone Application on the Intensity of Free Radical Destruction of Lipids and Intestinal Proteins in the Dynamics of Experimental Colitis Pubmed:35622259
Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease
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KSK504Hu01 ELISA Kit DIY Materials for Calprotectin (CALPRO) Main materials for "Do It (ELISA Kit) Yourself".