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Biotin-Linked Polyclonal Antibody to Asymmetrical Dimethylarginine (ADMA)

N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ADMA. It has been selected for its ability to recognize ADMA in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One. Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders PubMed: PMC3439466
Cytokine Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. Pubmed: 23834755
Neurol Res. Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. Pubmed:25475507
Int J Clin Exp Med. Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients Pubmed:25550968
Clin Respir J Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD PubMed: 26076870
Cytokine Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats PubMed: 26375520
Neurological Research Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats PubMed: 25475507
Scientific Reports Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study pubmed:27677852
clinica chimica acta Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. pubmed:27884754
The Journal of Physiology Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. pubmed:26840628
Angiogenesis Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). pubmed:29150732
Scientific Reports A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling Pubmed:29523851
Mechanisms of Ageing and Development Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories Pubmed:29605639
Nutrition, Metabolism and Cardiovascular Diseases Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns Doi: 10.1016/j.numecd.2018.09.002
Biochemical and Biophysical Research Communications Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats Pubmed: 30454888
Brazilian Journal of Pharmaceutical Science Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
ACS Combinatorial Science Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway Pubmed: 30673277
International Journal of the Cardiovascular Academy Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats
Oxid Med Cell Longev The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells Pubmed: 32104540
Molecular Medicine Reports Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … Pubmed: 31746378
International Journal of Nephrology Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients Pubmed: 33163233
Journal of Clinical Medicine The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial Pubmed: 32344735
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls 33463628
WIENER KLINISCHE WOCHENSCHRIFT Non-functioning adrenal incidentalomas may increase toxic metabolites 34255169
pharmaceuticals Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna 34959638
Int J Biochem Cell Biol HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer Pubmed:35644470
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