≡

Multiplex Assay Kit for Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay)

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-89 86
EDTA plasma(n=5) 91-101 96
heparin plasma(n=5) 85-98 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-103% 95-102% 89-102% 88-97%
EDTA plasma(n=5) 85-99% 99-105% 92-105% 97-105%
heparin plasma(n=5) 87-101% 93-105% 96-104% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FGF1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095
24 Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. pubmed:28750927
Endocrine Connections Characterization of fibroblast growth factor 1 in obese children and adolescents Pubmed:29991637
Molecular Medicine Reports Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditions Pubmed: 32323787
Annals of Agricultural and Environmental Medicine Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228
World J Hepatol Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis Pubmed:35070009
Catalog No. Related products for research use of Ovis aries; Ovine (Sheep) Organism species Applications (RESEARCH USE ONLY!)
RPA032Ov01 Recombinant Fibroblast Growth Factor 1, Acidic (FGF1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA032Ov01 Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) WB; IHC; ICC; IP.
SEA032Ov ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA032Ov Multiplex Assay Kit for Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CGA032Ov01 cDNA of Fibroblast Growth Factor 1, Acidic (FGF1) PCR; Protein Expression; etc.