Packages (Simulation)
Reagent Preparation
Image (I)
Image (II)
Certificate
Multiplex Assay Kit for Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay)
SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA101Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
For more details, please contact local distributors! US$ 821 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 79-105 | 91 |
EDTA plasma(n=5) | 95-104 | 98 |
heparin plasma(n=5) | 86-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 3 (MMP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-101% | 98-105% | 78-90% | 82-92% |
EDTA plasma(n=5) | 97-105% | 89-96% | 82-92% | 91-102% |
heparin plasma(n=5) | 90-101% | 79-101% | 88-97% | 95-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:MMP3) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
Magazine | Citations |
Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180 |
Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093 |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
China Pharmacy | Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats Source |
Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
physiological research | The skin remodeling in type 1 diabetes and insulin resistance animal models PubMed: 26047379 |
Physiol Res | The skin remodeling in type 1 diabetes and insulin resistance animal models. PubMed: 26047379 |
Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977 |
Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310 |
J Orthop Res. | Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661 |
Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977 |
Journal of Orthopaedic Research | Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661 |
Mediators of Inflammation | Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. pubmed:27433031 |
Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732 |
der Christian-Albrechts-Universität zu Kiel | Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration 1133074014:34 |
REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease E-ISSN: 1695-7504 |
REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular ISSN 1695-7504 |
Molecular Pharmacology | Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes 10.1124/mol.116.107300 |
Theranostics | Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis Pubmed:29774078 |
Chinese Journal of Integrative Medicine | Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway Pubmed: 30656598 |
Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… | |
PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166 |
ACS Biomaterials Science & Engineering | Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid … |
World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939 |
International Journal of Molecular Sciences | Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin Pubmed: 31652545 |
JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384 |
Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705 |
Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722 |
Alexandria Journal for Veterinary Sciences | The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. |
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588 |
journal biomedicine translational research | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic?¡ |
JOURNAL OF ANIMAL SCIENCE | Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis 34216471 |
Osteoarthritis Cartilage | A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments Pubmed:35618204 |
Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352 |
Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587 |