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Multiplex Assay Kit for Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA380Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.49-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.163 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-91 | 86 |
EDTA plasma(n=5) | 92-104 | 101 |
heparin plasma(n=5) | 88-104 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-91% | 98-105% | 92-99% | 87-101% |
EDTA plasma(n=5) | 92-103% | 87-95% | 95-105% | 78-95% |
heparin plasma(n=5) | 91-102% | 78-92% | 78-98% | 82-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:VIP) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Clinical Genetics | VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population Wiley: source |
Human immunology | Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy Pubmed: 23800435 |
Headache: The Journal of Head and Face Pain | CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372 |
World J Gastroenterol | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510 |
Cephalalgia? | Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine Pubmed:24847167 |
J Huazhong Univ Sci Technolog Med Sci. | Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients Pubmed:25318892 |
PLOS ONE | Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters Pubmed:25647228 |
PLOS ONE | Aqueous Fraction of PubMed: 25647228 |
Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921 |
World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732 |
Annals of Allergy, Asthma and Immunology | Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748 |
Cephalalgia. | Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233 |
Headache | No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine pubmed:27634731 |
Sci Rep. | Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. pubmed:28054652 |
BMC Complementary and Alternative Medicine | [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice 10.1186/s12906-017-1903-0 |
Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3 |
Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis Pubmed:28914594 |
Evidence-Based Complementary and Alternative Medicine | Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids Pubmed:29853946 |
Revista do Instituto de Medicina Tropical de São Paulo | Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients Pubmed: 30365640 |
Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement Pubmed: 30562932 |
Science of Food and Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer Pubmed: 30479041 |
MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice Pubmed: 32951099 |
Journal of Cystic Fibrosis | Disrupted local innervation results in less VIP expression in CF mice tissues Pubmed: 32600901 |
Biomedical Journal of Scientific & Technical Research | Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? |
Journal of tranditional chinese medical sciences | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
J Cyst Fibros | VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD) 34034984 |
Neurol Sci | Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study 34698942 |
Endocr J | A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience Pubmed:35644576 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA380Ra01 | Recombinant Vasoactive Intestinal Peptide (VIP) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA380Ra01 | Polyclonal Antibody to Vasoactive Intestinal Peptide (VIP) | WB; IHC; ICC; IP. |
MAA380Ra21 | Monoclonal Antibody to Vasoactive Intestinal Peptide (VIP) | WB; IHC; ICC; IP. |
CEA380Ra | ELISA Kit for Vasoactive Intestinal Peptide (VIP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA380Ra | Multiplex Assay Kit for Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA380Ra11 | ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP) | Main materials for "Do It (ELISA Kit) Yourself". |