≡

Multiplex Assay Kit for Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay)

FST; Activin-binding protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-89 85
EDTA plasma(n=5) 88-101 93
heparin plasma(n=5) 95-104 101
sodium citrate plasma(n=5) 82-101 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 94-105% 83-98% 80-99%
EDTA plasma(n=5) 91-99% 99-105% 79-94% 94-105%
heparin plasma(n=5) 82-101% 93-104% 93-105% 78-92%
sodium citrate plasma(n=5) 80-95% 80-95% 97-105% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FS) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Journal of cellular physiology Role of follistatin in muscle and bone alterations induced by gravity change in mice pubmed:28471505
Scientific Reports Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells Pubmed: 33203957
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA391Mu01 Recombinant Follistatin (FS) Positive Control; Immunogen; SDS-PAGE; WB.
RPA391Mu02 Recombinant Follistatin (FS) Positive Control; Immunogen; SDS-PAGE; WB.
PAA391Mu02 Polyclonal Antibody to Follistatin (FS) WB; IHC; ICC; IP.
PAA391Mu01 Polyclonal Antibody to Follistatin (FS) WB; IHC; ICC; IP.
CEA391Mu ELISA Kit for Follistatin (FS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA391Mu Multiplex Assay Kit for Follistatin (FS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.