≡

Multiplex Assay Kit for Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD286

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-95 89
EDTA plasma(n=5) 89-99 95
heparin plasma(n=5) 78-101 93
sodium citrate plasma(n=5) 98-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 87-103% 81-90% 79-102%
EDTA plasma(n=5) 89-103% 82-98% 96-104% 94-101%
heparin plasma(n=5) 80-103% 80-103% 90-97% 91-104%
sodium citrate plasma(n=5) 89-101% 78-98% 83-95% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TLR6) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Chinese Medical Journal Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297
Journal of Maternal-Fetal and Neonatal Medicine Soluble Toll-like receptor 1 family members in the amniotic fluid of women with preterm prelabor rupture of the membranes Informa: 14767058.2012.658463
PLoS One Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea Pubmed: 32069296
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA683Hu01 Recombinant Toll Like Receptor 6 (TLR6) Positive Control; Immunogen; SDS-PAGE; WB.
PAA683Hu01 Polyclonal Antibody to Toll Like Receptor 6 (TLR6) WB; IHC; ICC; IP.
MAA683Hu22 Monoclonal Antibody to Toll Like Receptor 6 (TLR6) WB; IHC; ICC; IP.
SEA683Hu ELISA Kit for Toll Like Receptor 6 (TLR6) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA683Hu Multiplex Assay Kit for Toll Like Receptor 6 (TLR6) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA683Hu01 ELISA Kit DIY Materials for Toll Like Receptor 6 (TLR6) Main materials for "Do It(ELISA Kit) Yourself"