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Multiplex Assay Kit for Amylin ,etc. by FLIA (Flow Luminescence Immunoassay)
IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA812Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.49-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.163 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 405
US$ 420
US$ 443
US$ 475
US$ 506
US$ 552
US$ 622
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Amylin ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-96 | 83 |
EDTA plasma(n=5) | 90-97 | 94 |
heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-97% | 88-99% | 81-105% | 95-105% |
EDTA plasma(n=5) | 80-89% | 78-91% | 92-105% | 94-104% |
heparin plasma(n=5) | 82-97% | 89-98% | 97-105% | 80-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
plos ONE | Differential Effect of Amylin on Endothelial-Dependent Vasodilation in Mesenteric Arteries from Control and Insulin Resistant Rats PubMed: 25807378 |
Nutrients | Glucagon Shows Higher Sensitivity than Insulin to Grapeseed Proanthocyanidin Extract (GSPE) Treatment in Cafeteria-Fed Rats 33810265 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA812Mu01 | Recombinant Amylin | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA812Mu02 | Recombinant Amylin | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA812Mu11 | BSA Conjugated Amylin | Immunogen; SDS-PAGE; WB. |
CPA812Mu21 | OVA Conjugated Amylin | Immunogen; SDS-PAGE; WB. |
PAA812Mu01 | Polyclonal Antibody to Amylin | WB; IHC; ICC; IP. |
PAA812Mu02 | Polyclonal Antibody to Amylin | WB; IHC; ICC; IP. |
PAA812Mu08 | Polyclonal Antibody to Amylin | WB; IHC; ICC; IP. |
CEA812Mu | ELISA Kit for Amylin | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA812Mu | Multiplex Assay Kit for Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA812Mu11 | ELISA Kit DIY Materials for Amylin | Main materials for "Do It (ELISA Kit) Yourself". |