Multiplex Assay Kit for Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay)

Pitocin; Syntocinon

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-101 82
EDTA plasma(n=5) 85-99 90
heparin plasma(n=5) 89-97 94
sodium citrate plasma(n=5) 79-98 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 94-101% 92-103% 85-101%
EDTA plasma(n=5) 90-105% 81-96% 99-105% 78-105%
heparin plasma(n=5) 85-99% 82-103% 95-102% 78-98%
sodium citrate plasma(n=5) 94-102% 83-95% 80-93% 85-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:OT) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
J Psychiatr Res.? Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals Pubmed:24411593
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study Pubmed:27143518
Progress in neuro-psychopharmacology & biological psychiatry Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect. pubmed:28093220
International Journal of Clinical and Experimental Medicine Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … files:ijcem0031358.pdf
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study pubmed:27143518
BMC Med. Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial. pubmed:28209155
Psychiatry Research Plasma oxytocin levels in major depressive and bipolar II disorders pubmed:28865715
BMC Medicine Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial 10.1186:s12916-017-0801-0
EUROPEAN ADDICTION RESEARCH The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users Pubmed:29902803
ASN Neuro . Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup Deprivation Pubmed: 32962418
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …
J. Pers. Med Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPB052Ge11 BSA Conjugated Oxytocin (OT) Immunogen; SDS-PAGE; WB.
CPB052Ge21 OVA Conjugated Oxytocin (OT) Immunogen; SDS-PAGE; WB.
PAB052Ge01 Polyclonal Antibody to Oxytocin (OT) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEB052Ge Mini Samples ELISA Kit for Oxytocin (OT) Enzyme-linked immunosorbent assay for Antigen Detection.
HEB052Ge High Sensitive ELISA Kit for Oxytocin (OT) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB052Ge ELISA Kit for Oxytocin (OT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB052Ge Multiplex Assay Kit for Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB052Ge11 ELISA Kit DIY Materials for Oxytocin (OT) Main materials for "Do It (ELISA Kit) Yourself".