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Multiplex Assay Kit for Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-93 88
EDTA plasma(n=5) 79-104 94
heparin plasma(n=5) 88-96 93
sodium citrate plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-101% 83-94% 78-98% 85-99%
EDTA plasma(n=5) 80-97% 93-101% 95-102% 94-105%
heparin plasma(n=5) 78-95% 87-99% 93-102% 98-105%
sodium citrate plasma(n=5) 79-96% 95-102% 82-94% 80-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SCT) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
SpringerPlus Weak cation exchange magnetic beads coupled with matrix-assisted laser desorption ionization-time of flight-mass spectrometry in screening serum protein markers in osteopenia Pubmed:27347465
Cell Secretin-Activated Brown Fat Mediates Prandial Thermogenesis to Induce Satiation Pubmed: 30449620
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
Novel non-adrenergic, endocrine mediators of brown adipose tissue thermogenesis
Nat Metab Secretin activates brown fat and induces satiation 34158656
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB075Mu02 Recombinant Secretin (SCT) Positive Control; Immunogen; SDS-PAGE; WB.
RPB075Mu01 Recombinant Secretin (SCT) Positive Control; Immunogen; SDS-PAGE; WB.
PAB075Mu02 Polyclonal Antibody to Secretin (SCT) WB; IHC; ICC; IP.
PAB075Mu01 Polyclonal Antibody to Secretin (SCT) WB; IHC; ICC; IP.
CEB075Mu ELISA Kit for Secretin (SCT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB075Mu Multiplex Assay Kit for Secretin (SCT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB075Mu11 ELISA Kit DIY Materials for Secretin (SCT) Main materials for "Do It (ELISA Kit) Yourself".