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Multiplex Assay Kit for Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay)

LGALS13; PLAC8; PP13; GAL13; Galectin 13; Galactoside-Binding Soluble Lectin 13; Placental tissue protein 13

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-101 95
EDTA plasma(n=5) 89-97 94
heparin plasma(n=5) 82-89 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 97-105% 99-105% 82-90%
EDTA plasma(n=5) 93-102% 79-95% 97-105% 82-96%
heparin plasma(n=5) 93-102% 94-103% 88-96% 83-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PP13) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Archives of Gynecology and Obstetrics Plasma PP13 and urinary GAGs/PGs as early markers of pre-eclampsia Pubmed:27161490
Pathophysiology Prediction of first-trimester preeclampsia: Relevance of the oxidative stress marker MDA in a combination model with PP-13, PAPP-A and beta-HCG Pubmed:29510888
J Matern Fetal Neonatal Med Prediction of preeclampsia using first trimester placental protein 13 and uterine artery Doppler Pubmed: 33198548
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB443Hu01 Recombinant Placental Protein 13 (PP13) Positive Control; Immunogen; SDS-PAGE; WB.
PAB443Hu01 Polyclonal Antibody to Placental Protein 13 (PP13) WB,IHC
MAB443Hu22 Monoclonal Antibody to Placental Protein 13 (PP13) WB; IHC; ICC; IP.
SEB443Hu ELISA Kit for Placental Protein 13 (PP13) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB443Hu Multiplex Assay Kit for Placental Protein 13 (PP13) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.