≡

Multiplex Assay Kit for Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay)

InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 98
EDTA plasma(n=5) 87-98 93
heparin plasma(n=5) 95-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 84-99% 95-104% 78-94%
EDTA plasma(n=5) 83-103% 79-99% 88-95% 79-103%
heparin plasma(n=5) 88-96% 87-96% 87-96% 88-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IP3) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Medicine Reports The association between the expression of PAR2 and TMEM16A and neuropathic pain Pubmed:29257338
Journal of Biological Chemistry OSBP-related protein 4L promotes phospholipase Cβ3 translocation from the nucleus to the plasma membrane in Jurkat T-cells Pubmed: 30237164
Acta Pharmacologica Sinica α 1-AR overactivation induces cardiac inflammation through NLRP3 inflammasome activation Pubmed: 31530901
Translational Research Exendin-4 inhibits small intestinal glucose sensing and absorption through repression of T1R2/T1R3 sweet taste receptor signalling in streptozotocin diabetic mice Pubmed:35385790
Nat Nanotechnol A pyroptosis nanotuner for cancer therapy Pubmed:35606443
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CEC037Ge ELISA Kit for Inositol Triphosphate (IP3) Enzyme-linked immunosorbent assay for Antigen Detection.
CCC037Ge CLIA Kit for Inositol Triphosphate (IP3) Chemiluminescent immunoassay for Antigen Detection.
LMC037Ge Multiplex Assay Kit for Inositol Triphosphate (IP3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.