≡

Multiplex Assay Kit for Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay)

BMP11; Bone morphogenetic protein 11

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-105 93
EDTA plasma(n=5) 81-93 88
heparin plasma(n=5) 97-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-102% 89-104% 79-94% 78-91%
EDTA plasma(n=5) 91-104% 85-94% 80-96% 84-98%
heparin plasma(n=5) 90-101% 84-97% 78-96% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:GDF11) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oncotarget Splenocytes derived from young WT mice prevent AD progression in APPswe/PSENldE9 transgenic mice PubMed: 26317549
Endocrinology TGFβ Superfamily Members Mediate Androgen Deprivation Therapy-Induced Obese Frailty in Male Mice pubmed:27611336
International Journal of Clinical and Experimental Pathology GDF11 is increased in patients with myelodysplastic syndrome files:ijcep0024948.pdf
European Review for Medical and Pharmacological Sciences GDF11 does not improve the palmitate induced insulin resistance in C2C12. pubmed:28485800
CyberLeninka РОЛЬ ДИФФЕРЕНЦИРОВОЧНОГО ФАКТОРА РОСТА 11 (GDF11) В РЕГУЛЯЦИИ ЛИПИДНОГО ОБМЕНА И КАРДИОГЕМОДИНАМИЧЕСКИХ ФУНКЦИЙ … Cyberleninka:Source
Hematology GDF11 is increased in patients with aplastic anemia Pubmed: 30727851
J Cell Mol Me GDF11 prevents the formation of thoracic aortic dissection in mice: Promotion of contractile transition of aortic SMCs 33764670
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839
Oxidative Medicine and Cellular Longevity Low-Intensity Exercise Routine for a Long Period of Time Prevents Osteosarcopenic Obesity in Sedentary Old Female Rats, by Decreasing Inflammation and … 34336096
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC113Hu02 Recombinant Growth Differentiation Factor 11 (GDF11) Positive Control; Immunogen; SDS-PAGE; WB.
RPC113Hu01 Recombinant Growth Differentiation Factor 11 (GDF11) Positive Control; Immunogen; SDS-PAGE; WB.
PAC113Hu02 Polyclonal Antibody to Growth Differentiation Factor 11 (GDF11) WB; IHC; ICC; IP.
PAC113Hu01 Polyclonal Antibody to Growth Differentiation Factor 11 (GDF11) WB; IHC; ICC; IP.
MAC113Hu21 Monoclonal Antibody to Growth Differentiation Factor 11 (GDF11) WB
SEC113Hu ELISA Kit for Growth Differentiation Factor 11 (GDF11) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC113Hu CLIA Kit for Growth Differentiation Factor 11 (GDF11) Chemiluminescent immunoassay for Antigen Detection.
LMC113Hu Multiplex Assay Kit for Growth Differentiation Factor 11 (GDF11) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.