≡

Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay)

FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-99 94
EDTA plasma(n=5) 89-103 99
heparin plasma(n=5) 80-101 79

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 78-89% 89-103% 83-94%
EDTA plasma(n=5) 82-101% 84-101% 89-101% 94-103%
heparin plasma(n=5) 96-103% 78-95% 78-104% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FASN) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121
Oncogenesis. Diet-induced alteration of fatty acid synthase in prostate cancer progression Pubmed:26878389
Animal Science Journal Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941
life sciences Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma Pubmed: 30914315
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
bmc research notes The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC470Ra01 Recombinant Fatty Acid Synthase (FASN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC470Ra01 Polyclonal Antibody to Fatty Acid Synthase (FASN) WB; IHC; ICC; IP.
MAC470Ra21 Monoclonal Antibody to Fatty Acid Synthase (FASN) WB; IHC; ICC; IP.
SEC470Ra ELISA Kit for Fatty Acid Synthase (FASN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC470Ra Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.