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Multiplex Assay Kit for Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay)
CN1; CPGL2; Beta-Ala-His Dipeptidase; Metallopeptidase M20; Carnosinase 1; Serum carnosinase; Glutamate Carboxypeptidase-Like Protein 2
(Note: Up to 8-plex in one testing reaction)
- Product No.LMF388Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, cerebrospinal fluid and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
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Result
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-101 | 80 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 82-101 | 96 |
| sodium citrate plasma(n=5) | 78-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-102% | 86-99% | 81-98% | 96-103% |
| EDTA plasma(n=5) | 80-103% | 85-99% | 81-98% | 78-95% |
| heparin plasma(n=5) | 86-97% | 87-96% | 94-102% | 80-95% |
| sodium citrate plasma(n=5) | 88-96% | 91-98% | 84-96% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CNDP1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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| Magazine | Citations |
| PLoS One. | Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis PubMed: 23029420 |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135 |
| Journal of Proteomics | Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approach Pubmed: 30605725 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF388Hu01 | Recombinant Carnosine Dipeptidase 1 (CNDP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF388Hu01 | Polyclonal Antibody to Carnosine Dipeptidase 1 (CNDP1) | WB; IHC; ICC; IP. |
| MAF388Hu21 | Monoclonal Antibody to Carnosine Dipeptidase 1 (CNDP1) | WB; IHC; ICC; IP. |
| SEF388Hu | ELISA Kit for Carnosine Dipeptidase 1 (CNDP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF388Hu | Multiplex Assay Kit for Carnosine Dipeptidase 1 (CNDP1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |