Packages (Simulation)
Image (I)
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AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Mouse Anti-Human C5a Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Monoclonal Antibody to Complement Component 5a (C5a)
- Product No.MAA388Hu25
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)F5
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
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For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against C5a. It has been selected for its ability to recognize C5a in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297 |
| Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845 |
| The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471 |
| Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587 |
| Journal of Orthopaedic Research | Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742 |
| SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977 |
| American Journal fo Reproductive Immunology | Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155 |
| Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247 |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358 |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602 |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
| Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439 |
| Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586 |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106 |
| Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296 |
| Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479 |
| Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976 |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004 |
| Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855 |
| Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … |
| Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478 |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006 |
| Modulation of C5a-C5aR interactions against murine mammary cancer cell line | |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |