Monoclonal Antibody to Estradiol (E2)

17B-Estradiol; Oestradiol; Beta-Estradiol

SPECIFITY

The antibody is a mouse monoclonal antibody raised against E2. It has been selected for its ability to recognize E2 in immunohistochemical staining and western blotting.

USAGE

Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BioMed research international Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis PubMed: PMC3678416
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
Repositório Digital Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas Lume: Source
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source
Academic Sciences Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source
Stem Cells Int. Cell Therapy for Chemically Induced Ovarian Failure in Mice Pubmed:Pmc4274854
Reproductive Toxicology Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190
Phytomedicine. Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice Pubmed:24932975
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886
Reprod Toxicol In utero PubMed: 25530038
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940
Environ Toxicol Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782373
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118
Reproduction Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats PubMed: 26556892
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642
Biology of Reproduction Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362
Clinical and Experimental Hypertension Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507
PLoS Genet. A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770
Scientific Reports The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659
Journal of Functional Foods Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
Environmental toxicology and pharmacology Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Interact Cardiovasc Thorac Surg Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666
Reproductive Sciences Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204
Oncotarget Rcan2 and estradiol independently regulate body weight in female mice pubmed:28624805
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 10.5897/AJEST2016.2234
Journal of Orthopaedic Research The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X
Nanotheranostics The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593
Molecular Medicine Reports Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells pubmed:28656272
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract
Stem Cell Research & Therapy The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Aquatic Toxicology Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations Pubmed:29775925
Journal of Ethnopharmacology Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model Pubmed:29705515
Inflammation Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male Pubmed:29737476
European Journal of Neuroscience Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in … Pubmed:29205599
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035
Medical Science Monitor Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Pubmed:29674607
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
journal of molecular neuroscience Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches Pubmed: 30600432
Heliyon Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
Evidence-Based Complementary and Alternative Medicine iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
Biomedicine & Pharmacotherapy G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia … Pubmed: 30784936
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve
Biology of Reproduction G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … Pubmed: 31034039
Biomedicine & pharmacotherapy Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapy Pubmed: 31207575
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554
Experimental Neurology Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Pubmed: 31377402
Oxidative medicine and cellular longevity Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen Therapy Pubmed: 31428228
Journal of Ovarian Research Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718
Cellular Signalling Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro Pubmed: 31704004
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938
Neuroscience Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats Pubmed: 31805254
Open Access Maced J Med Sc Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model Gastritis Pubmed: 31949507
Estradiol treatment during perinatal development alters Pubmed: 31966342
science of the total environment Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells Pubmed: 32334352
molecular nutrition & food research Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1 Pubmed: 32239630
Chemosphere Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) Pubmed: 33011509
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats Pubmed: 32790534
ARCHIVES OF GYNECOLOGY AND OBSTETRICS MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043
Antioxidants (Basel) Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356
Andrologia Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty Pubmed: 32478927
Neurotoxicology Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice Pubmed: 32407858
archives of toxicology Effect of estrogen-active compounds on the expression of RACK1 and immunological implications Pubmed: 32328699
Heliyon Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488
Iranian Journal of Basic Medical Sciences Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
biorxiv The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice
Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle
J Steroid Biochem Mol Biol Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development 33465420
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655
JOURNAL OF ETHNOPHARMACOLOGY BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368
Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice
Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331
Eur J Pharmacol 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice 34822791
IBRO Neuroscience Reports Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice 34746914
Nutrients Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459
Nat Commun TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice Pubmed:35177657
Appl Biochem Biotechnol ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary Pubmed:35138554
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA461Ge11 BSA Conjugated Estradiol (E2) Immunogen; SDS-PAGE; WB.
CPA461Ge21 OVA Conjugated Estradiol (E2) Immunogen; SDS-PAGE; WB.
CPA461Ge31 KLH Conjugated Estradiol (E2) Immunogen; SDS-PAGE; WB.
PAA461Ge01 Polyclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA461Ge71 Biotin-Linked Polyclonal Antibody to Estradiol (E2) WB; IHC; ICC.
LAA461Ge81 FITC-Linked Polyclonal Antibody to Estradiol (E2) WB; IHC; ICC; IF.
MAA461Ge26 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge21 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge22 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge29 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge28 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA461Ge27 Monoclonal Antibody to Estradiol (E2) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA461Ge ELISA Kit for Estradiol (E2) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA461Ge Instant ELISA Kit for Estradiol (E2) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA461Ge Mini Samples ELISA Kit for Estradiol (E2) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA461Ge CLIA Kit for Estradiol (E2) Chemiluminescent immunoassay for Antigen Detection.
LMA461Ge Multiplex Assay Kit for Estradiol (E2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA461Ge11 ELISA Kit DIY Materials for Estradiol (E2) Main materials for "Do It (ELISA Kit) Yourself".